Microscopy · Neurodegeneration

Detecting fluorescence hotspots in yeast cells

Quantifying spatial intensity patterns associated with cellular stress and protein aggregation.

  • Fluorescence
  • OpenCV
  • Segmentation
  • Scientific visualisation

Case study

01 · The question

Challenge

Biologically meaningful hotspots must be separated from imaging noise and cellular background.

02 · The work

Approach

I implemented image preprocessing, segmentation, hotspot detection, and statistically interpretable visualisation in Python and OpenCV.

03 · The value

Impact

The work supported biological interpretation and led to a peer-reviewed IEEE conference publication.

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