PhD research · Biophysics + AI

Counting single bacteriophages with interpretable deep learning

A density-map framework connecting TIRF microscopy predictions to molecular binding kinetics.

  • TIRF microscopy
  • U-Net
  • Density maps
  • HPC
  • Biophysics

Case study

01 · The question

Challenge

Single bacteriophages appear as crowded, variable fluorescence signals. Reliable counting must remain robust across imaging conditions while retaining a meaningful relationship to the underlying molecular process.

02 · The work

Approach

I developed U-Net-based density prediction workflows, controlled synthetic data, automated HPC training pipelines, and validation strategies grounded in Poisson statistics and Langmuir binding models.

03 · The value

Impact

The work links pixel-level inference with estimates of antigen–antibody affinity, creating a path from computer vision output to interpretable biophysical measurement.

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